FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11454027
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2173851.897891457737964No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA587020.5125009745480782No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA494800.4319878065592127No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400590.3497372583459075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161030.1405881093173606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCT31950.018.86666520
TACTCGG41250.017.8663738
CTACTCG44100.017.7593437
TTAGCCG50550.017.3338871
TAGCCGG56000.017.2854252
ACTCGGG42100.017.24436839
CTCGGGA39700.017.23396940
GCGCCTG44150.017.19110721
CCTAGTC27200.016.98501216
GGGCGCC28600.016.76895119
GGGTTGC28850.016.3948756
TGGCGGG39800.016.30626115
GGTGGCG59800.015.70876913
TCGGGAG44000.015.49974241
GTGGCGG42400.015.35823414
GGAGGCT167500.015.01228744
CTAGCCG5450.014.9464921
CGGGCGT27750.014.8249786
GCTACTC124250.014.71363736
GCGGGCG22200.014.66642217
Produced by FastQC (version 0.11.5)