FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9340644
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA1350.035.862251
CGGTATG10100.032.2469751
CGGTGTA12550.031.5629391
GCGCCTA10350.031.4680611
GCGTACA9050.031.3682121
GCGTACT7650.031.35551
CGGTATA12350.031.1831341
GCGCCAA11600.031.1124731
CGGCGTA2200.031.0089951
GCGTAAC5000.030.8089371
GCGCCTT16950.030.7699851
CGGTAAT11600.030.7330531
GCGTCTA8850.030.585141
CGGTATT17950.030.5269581
CGGTCTT15250.030.4481771
GCGTATA10800.030.3606571
GCGCATA7550.030.3134271
GCGCATT12000.030.2587761
GCGTCCA12050.030.133221
GGCCCAT76800.030.0868531
Produced by FastQC (version 0.11.5)