FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9340644
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190450.20389386427745237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAAA295950.011.7071
CAAAAAA292600.09.2773571
TAGCCGG18550.08.5383122
CGTATTA3157.441445E-58.3801951
CGGGCGT8900.08.1565666
GGATCGT3604.0523235E-57.943811428
TTAGCCG17000.07.76400381
GGCGCCT11700.07.712048520
TACTCGG14000.07.542175338
CTACTCG14950.07.504338737
CGAACTT3552.9774342E-47.4359481
CGAACAA4459.2314185E-67.415061
CGGTCAG4304.690259E-57.1621431
GCTACTC42450.07.09947636
ATCGTTC4355.460946E-57.079895530
CGGTATT3450.00179205927.0138591
GCGCCTG15250.06.782655721
GATCGTT4559.8489654E-56.76869229
TAAAAAA291650.06.70537661
CGATGCT4601.1362473E-46.6950471
Produced by FastQC (version 0.11.5)