FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9246404
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA1950.037.251181
GCGTACT6450.034.1272281
CGGTACA11850.033.993311
CGGCGAT3400.033.0180931
CGGTCCG1200.033.018091
GCGCGTT3500.032.074721
CGGTATA13950.031.8740921
GCGTACA9700.031.7699831
CGCGTAT1600.031.6413082
CGGTATT16600.031.559461
CGGTATG8800.031.267131
CGGTACT8900.031.1631411
CGGTCTA7700.031.1599271
GCGTGTA10550.031.0881231
GCGCCTT17900.030.8660741
CGGTGTA11850.030.463951
GCGTCCA11650.030.42011
GCGTCTT13900.030.2467881
GCGTCTA8450.029.9572411
CGGCGTT3100.029.8227921
Produced by FastQC (version 0.11.5)