FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6322014
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG2200.034.9997526
CGGTATA9050.034.773191
GCGCCTT12500.034.330621
GCGCGTT1800.034.232811
ACGGCGA2250.034.2219857
CGGTATG5950.034.0273321
GCGCGGA850.033.657471
CCGTACA4000.033.0102121
GCGTATA7400.033.0102121
CGGTATC4900.032.7856521
CGGTATT12650.032.705771
GCGCATT6950.032.297761
CGGTCTA5550.032.1180421
GCGTAAC2400.032.0932661
GCGACTT5800.031.8719311
GCGTACT4850.031.7624031
CGGTCCA6550.031.5822891
CGGTACA6900.031.5749861
GCGCCCT9900.031.565321
CGGTCTG7500.031.396381
Produced by FastQC (version 0.11.5)