FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6322014
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114570.18122389479048923No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86360.1366020385275958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCG757.027302E-826.39807738
GGCTCGA2700.021.18364131
TCGAATC3150.018.8557734
TACTCGG26800.016.90921638
CTAGCCG4700.016.8498361
GCTCGAA3400.016.82230632
TAGCCGG36250.016.567072
ACTCGGG25900.016.562539
CTACTCG28250.016.50853237
GCGACCA800.00199057216.4987989
GGAGGCT105150.016.3640844
CTCGGGA24200.016.36244440
TTAGCCG33650.016.1474131
TACTCCG2900.015.92987438
GCGGGCG12300.015.92320717
GGCGCCT22900.015.27943620
TGGCGGG25500.015.26947715
CGGTCGG1301.3717121E-515.22966139
GCGCCTG28450.015.08335721
AGCCGGG41400.014.7718723
Produced by FastQC (version 0.11.5)