FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6260705
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110700.1768171475896085No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83800.13385074045175424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAA2800.021.99835432
GGCTCGA2900.020.48122831
CTCGGGA25300.018.5199140
TCCCGCG600.007420383418.331233
ACTCGGG27500.018.23790739
TACTCGG28500.018.21545438
TCGGGAG25400.017.84075741
GGCGCCT24350.017.5345320
CGAATCT3400.017.46886335
ACGGTCG1154.283327E-617.21541838
CTACTCG30800.016.9266337
TCGAATC3800.016.78781734
TAGCCGG38500.016.7409362
TTAGCCG37050.016.6242731
GCGCCTG29200.016.35566921
GGAGGCT97900.016.11604544
GCGTTGA5800.015.9292078
GGGCGCC20200.015.79821719
CTAGCCG3550.015.491181
CACGGAA4850.015.42091316
Produced by FastQC (version 0.11.5)