FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8466456
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT3200.033.6958431
CGGTATA12050.033.2364461
GCGTACT5700.032.8151441
GCGCCTT16700.032.8105161
CGGTCTT15800.032.590351
GCGACCA8900.031.895541
GCGTACA7650.031.354171
GCGCATA6450.031.0464481
CGGTAAT12550.031.0355721
CGGTGTA10250.030.9149741
GCGTATA10900.030.8883821
CGGACCG1000.030.807631
GGGCGTT9250.030.6886831
CGGTCCT12650.030.6162781
GCGCGAC1150.030.6162761
CGGTATG9450.030.5049061
GCGCATT10500.030.3884771
GCGACCC5650.030.3792081
CGGTCTG10900.030.2827281
GCGTCTT12700.030.1491971
Produced by FastQC (version 0.11.5)