FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8466456
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210970.24918336550736223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAAA290300.012.0484991
CGGTCTT2701.2113458E-59.7768811
CAAAAAA270950.09.2229971
TACTCGG13400.09.02902338
CGATATG1950.0068931929.0248141
CTACTCG13750.08.95917837
CTAGCCG2300.00273480698.6079071
CGGACAA3106.1718E-58.5153491
TAGCCGG17050.08.5153482
GCGCCTG15000.08.36288821
CGCAATT2800.00182445957.85642241
GCTACTC38050.07.746990736
TTAGCCG16550.07.7092631
GGCGCCT12050.07.670698620
GTAGTCC35200.07.5619427
GGTGGCG21150.07.488675613
ACTCGGG15850.07.355792539
TAAAAAA278050.07.19948341
CAGCTAC65000.07.03935534
GGGCGCC10100.06.972705419
Produced by FastQC (version 0.11.5)