FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8345207
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA1450.037.9512631
CCGCGTA1150.036.367211
GCGCGTT3050.034.6414151
CGGTATG8200.033.554471
GCGCCTT15850.033.3300671
CGGTGTA9150.033.198021
GCGACTA6000.033.0175971
CGGTATA10850.032.8654441
GCGCATT10150.032.7465171
CGGTACA8000.032.742451
GCGCGAA1300.032.1709941
GCGTGTA9000.032.0392951
GCGACCA9250.031.6493021
CGGTATC8300.031.5589891
GCGCCTA9450.031.4453321
CGGTCTT15050.031.1528191
GGGCGTT8500.030.8164231
CGGCGTA1800.030.571851
GCGTCTT13650.030.4777851
CGGTCCT11400.030.3144041
Produced by FastQC (version 0.11.5)