FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8345207
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA197070.2361475275568359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAAA281050.012.4837931
GGCGCCT10100.09.58838520
TAGCCGG16350.09.5523462
CAAAAAA263600.09.2962811
CGGTTAC2000.0084855698.7989221
TTAGCCG15250.08.7989211
CGGACTT3502.8505281E-58.1704261
CGACAGG3553.4042663E-58.055351
GTAGTCC31700.07.980524527
CGGAAAC3401.8093214E-47.7637541
CTACTCG13050.07.753838537
GCGGATC9550.07.601162412
TAGTCCC37700.07.46898328
GGTGGCG18750.07.391094713
GGGCGCC8353.6379788E-127.380498419
GCGCCTG14100.07.33656721
CGATTAA3300.00111919927.3324341
TACTCGG13250.07.30476538
GTGGCGG13550.07.14303614
CGGAACT3704.7904826E-47.13426071
Produced by FastQC (version 0.11.5)