FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8162171
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG5150.036.3097726
CGGTATA11750.033.907831
CGGTGTA9000.033.507141
ACGGCGA5600.033.392027
CGGTACA8850.032.8314441
CGGTATT14550.032.8288761
GCGCCTT18100.032.592341
CGGCGTA1850.032.1256031
CGGACCA7450.031.6145341
GCGCATT9850.031.5095481
GCGTCCT11000.031.417111
CGGTCTA8200.031.407351
CGGTATG10400.031.3247531
GCGCCTA10700.031.269371
GCGCCCT14150.031.2679141
CGGTCTT19450.031.2355211
GCGTATA8600.031.2263091
GCGGGTA5300.031.1490421
CGGTCCA7800.031.0425471
GCGCGTT2000.030.8167861
Produced by FastQC (version 0.11.5)