FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8162171
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144360.1768647091564242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGA2200.018.99895931
GGCGCCT27200.018.2055620
CGGTCGG1355.5444616E-817.92384339
TACTCGG35750.017.22876738
ACGGTCG1154.2837055E-617.21539138
GGAGGCT134850.016.97012344
CTACTCG37500.016.77671837
ACTCGGG35250.016.66189639
CTCGGGA32200.016.60106540
GGGCGCC23650.016.4714819
TTAGCCG47300.016.3700921
GCGCCTG36050.016.36138321
TAGCCGG51850.016.1638872
GCGTTGA7500.016.1313638
TCTGCGT8350.015.543015
TCGGGAG34550.015.28089241
TGCGTTG8150.015.1147167
GCGCCTA2800.014.934340521
TGGCGGG36000.014.78708115
CTCTGCG8950.014.7467994
Produced by FastQC (version 0.11.5)