FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8008425
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA8150.033.479221
GCGCCTA9300.033.361651
GCGCCTT19200.033.235951
GCGACCA7350.032.6325071
GCGACTT7900.030.6391641
CGGTATT13450.030.5936011
CGGACCA8500.030.547411
CGGTCTT15450.030.4787121
CGGTACT7400.030.3305151
GCGCGTT2700.030.15431
GCGCATA6000.030.0728021
CGGTCCT13650.029.661731
GCGCCCT18550.029.6556471
GCGACTG7650.029.6269631
GGCCCTT84200.029.5309661
GGCCCAT79950.029.3943671
CGGTATA10150.029.2670571
GGCCCCC94450.029.098581
GCGCATT10000.029.0459271
GCGTCTT11900.029.0311321
Produced by FastQC (version 0.11.5)