FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8008425
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA502870.6279262152046127No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160370.2002516100231943No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA142840.1783621623477775No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120600.1505914084230045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG20650.014.6074051
TAGCCGG21850.014.0959312
CCTAGTC14950.012.50812516
CTACTCG17750.012.27019137
GGGTTGC18450.011.6854176
TACTCGG17500.011.565495538
CTAGTCT16750.011.29530817
TCCTAGT17550.011.28183715
TCGCGCA1400.006219745610.99979317
GGCGCCT14500.010.92393220
GTAGTCC47550.010.82629427
GCTACTC50900.010.5459736
GCGCCTG20950.010.50099621
GGGCGCC14350.010.424890519
GTTGCAA22800.010.3243678
CAGCTAC82100.010.20930834
AGCTACT84000.010.18790335
TGTAGTC55700.010.07162426
CCTGTAG56600.09.87260524
TAGTCCC52550.09.75433428
Produced by FastQC (version 0.11.5)