FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7825430
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA10050.033.0643921
CGGTATG7550.032.9367831
GCGACTC5100.032.36241
GCGCCTA8450.032.2934651
CGGTACT6900.032.2123151
GCGACCA7900.031.7561191
GCGCCTT19200.031.7488671
GCGACTA4800.031.6342491
GCGTACT4550.031.437761
GCGACTT6600.031.3424971
GCGTCTA5550.030.9279631
GCGTATC4500.030.8090081
CGGTACA8650.030.2747461
GCGCGTT2550.030.2049081
CGGACCA8350.030.0447121
CGGTCTA7700.030.0087721
GCGCATT9750.029.7933251
CGGTCCT13850.029.5537661
GGCCCAT70150.029.5196781
CGGCGTA1650.029.3419131
Produced by FastQC (version 0.11.5)