FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7825430
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA467360.5972323565605979No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA152090.19435353712192174No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA136880.17491690552468045No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114140.1458577995075031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG20500.015.1402881
TAGCCGG22600.014.6015562
GGCGCCT12450.013.60622620
CCTAGTC13850.013.0250816
CTACTCG17000.012.423346537
GCTACTC48250.011.94593836
GTAGTCC42450.011.60885427
TACTCGG17350.011.53873538
GCGCCTG18800.011.35089721
AGCCGGG30300.011.3265673
CTAGTCT15450.011.24902617
TCCTAGT16400.011.13398315
CAGCTAC77350.011.09227434
TGTAGTC49400.011.08897726
CGGGCGT11000.010.9998396
TAGTCCC47050.010.75442728
GGGTTGC17050.010.5804896
CCAGCTA81450.010.47989633
AGCTACT79900.010.38032335
CCTGTAG53700.010.36490524
Produced by FastQC (version 0.11.5)