FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9709578
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA796480.8203034158642116No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA295770.3046167402949953No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA269710.27777726282233894No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA212140.2184852935936042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGC1100.001019013513.99980434
TTAGCCG26350.013.7869021
TAGCCGG28950.013.6025722
CTACTCG23300.013.12421237
GCGCCTG22650.012.72381721
CCTAGTC17500.012.57118716
GCGCGCC3153.092282E-1112.57118719
ACGCGCC1751.6001979E-512.57118719
TACTCGG23700.012.53140538
GGCGCCT16350.011.97530620
CGCGCCT4650.011.82772920
GTAGTCC59750.011.78220127
CGGGCGT13300.011.5787266
GCTACTC65750.011.07507236
ACTCGGG27450.010.97975339
GGGTTGC21450.010.9741496
TAGTCCC65300.010.88187428
CAGCTAC103500.010.86168334
GGTGGCG34250.010.85526713
AGCTACT105550.010.79662635
Produced by FastQC (version 0.11.5)