FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9506050
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTT22550.034.156421
GCGCGTA1100.032.0094451
CGGTACA9650.031.6984751
CGGTATA12450.031.4631021
GCGACCA9800.031.4378531
CGGTCTT18550.031.4378531
GCGACTT8000.031.3592551
GCGCGTT2250.031.2981261
CGGTCCT16400.031.2653261
GCGACCC8400.030.9138871
CGGGGTA9050.030.8820421
GCGTCTT14450.030.7634031
CGGACCA9350.030.5972671
GCGCCCT22400.030.4554181
GCGTCCT16950.030.380651
GCGCCTA10450.030.1141511
CGGTGTA10700.030.0275551
CGGACTG9950.029.8580591
GGCCCTT110000.029.848811
GCGCATA6250.029.576731
Produced by FastQC (version 0.11.5)