FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8898629
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT2800.033.7897451
CGCGTAA353.2172425E-431.4323232
CGGACCA8600.030.9571841
GCGCCTA10200.030.631111
CGGTACT6850.030.5145911
CGGTATT16100.030.4756891
CGGTCTT17650.030.4172291
CGGTATA12250.030.3546451
GCGTACT6100.030.2986981
CGGTGTC9700.030.168551
GCGCCTT19850.030.1496941
CGGTAAC5350.030.0222761
CGGTATG9800.029.8607061
CGGTCCT12900.029.8485261
GCGCGTA1550.029.810011
GCGTCCT13550.029.5533431
CGGTATC7900.029.5225141
GGCCCTT87850.029.4286731
GCGCATT10600.029.2676471
GCGGGTT11000.029.2034871
Produced by FastQC (version 0.11.5)