FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8704395
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA11250.032.079281
GCGCCTT18400.032.0516431
GCGGGTA6150.031.4876941
GCGCGTA2100.031.4365771
CGGTACA9450.031.4365771
CGGTATT16550.031.3795931
GCGTACT6150.031.1298791
GCGCCTA10350.031.0417231
CGGTGTC10600.030.5172061
CGGTCCT12350.030.4692971
CGGTACT8250.030.4077451
CGGACCG1750.030.1791171
CGGCGTA1400.029.864751
CGGTGTA9500.029.6496581
CGGTATG8600.029.4260981
GGCCCAT78200.029.0687871
GCGTATC6100.028.859811
GCGACCT9550.028.8031461
CGGTCTT18050.028.5280421
GCGACCA7750.028.3943311
Produced by FastQC (version 0.11.5)