FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7959678
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA1450.036.4216421
GCGCCTT16700.034.654171
CGGACCG1100.034.0073281
CGGTACT7150.033.545691
GCGCGTT2650.032.3843351
CGGTATA9800.032.108961
CGGTACA7200.032.090251
GCGTATC4750.031.9647831
CGGTCCT13750.031.846861
GCGCATA6050.031.643181
CGGTATT15850.031.2370131
GCGACTG6500.030.8066351
GCGACCG1500.030.8066351
CGGTCTG11900.030.6956881
CGGTATG8900.030.658291
CGGACCA8550.030.626481
CGGCGTA1800.030.562141
CGGTCTT15650.030.5113661
CGGTAAT8300.030.4884971
GCGCCTA8650.030.0180281
Produced by FastQC (version 0.11.5)