FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7959678
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA971781.2208785330260847No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370420.46537058408644166No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA333620.41913755807709807No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA272540.3424007855594159No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTC17450.018.15433916
TAGCCGG27800.017.0933742
TTAGCCG25700.017.0483631
GGCGCCT15650.017.0092220
TACTCGG22650.016.51178738
CTACTCG23700.015.96590337
TCCTAGT21050.015.67662315
GGGTTGC21600.015.1756016
GCGCCTG21500.015.14386221
ACTCGGG23550.015.13343339
CGGGCGT13150.014.7221186
CTCGGGA23200.014.50830940
CTAGCCG2358.1490725E-1014.0535521
CTAGTCT22500.013.98192317
AGCCGGG35900.013.4816063
GCTACTC61900.013.46982636
CGGATAG1801.4356938E-613.4549751
GGGCGCC14750.013.12514319
TGGCGGG20850.013.083652515
GTAGTCC60300.012.91530827
Produced by FastQC (version 0.11.5)