FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7827414
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCA7150.034.163641
CGGCGAT1750.033.9525871
CGGCGTA1450.033.3888781
GCGCCTT15250.033.0455361
CGGTACG1000.033.009461
CGGTCTG10450.032.6409341
CGGTATG8750.032.4435841
CGCGGTA551.3719182E-732.0091741
CGGTACA6500.031.8245071
CGGTCCA9100.031.6794091
CGACCAA3550.031.612072
CGGTCCT12550.031.387481
GCGTATT9050.031.3681071
CGGTGTA9000.031.2978611
GGGCGTT6500.031.1473881
CGGTATC6150.031.1308731
GCGCATA5750.031.0001891
GCGACTG6900.030.617471
CGGTCTT15900.030.5873811
CGGTATA9500.030.5771851
Produced by FastQC (version 0.11.5)