FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7827414
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA917921.1726989271297008No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA359240.4589510660864495No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA324720.41484965532677837No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA264710.33818321095575116No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG19950.017.4232238
CCTAGTC16900.017.31327216
CTACTCG21150.016.85073737
ACTCGGG21850.015.20346339
CTAGTCT20050.015.03212717
TTAGCCG25350.015.0239761
TCCTAGT19750.014.70351115
TAGCCGG26600.014.55629352
GGGTTGC20300.014.0883966
GCGCCTG20450.013.55474921
GGCGCCT15800.013.50606620
TGGCGGG20250.013.47134315
GGTGGCG28400.013.40114813
CTCGGGA20950.013.33634140
CGGGCGT12800.013.0622446
GTGGCGG21550.012.45451414
GCTACTC59050.012.368937536
GAAAAAA319750.012.2484781
AGCCGGG35400.011.8698253
CAGCTAC95300.011.86545734
Produced by FastQC (version 0.11.5)