FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10131267
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTT20650.032.927241
GCGCCTA11350.031.795441
CGGCGTA1600.031.6318591
GCGCGTA1600.031.6318591
CGGTCTT19850.031.5937521
CGGTATA15200.031.5594751
CGGTATT17250.030.9980281
GCGTACA7950.030.723641
CGGACCA10750.030.7043321
GCGTATC5650.030.3782671
CGGTCTA9550.030.1845571
GCGTCTT17000.029.641721
GCGCCCT18350.029.6195031
CGGTACT8650.029.5092891
GCGACTG9100.029.5009041
GCGCGTT2400.029.3396971
GGGCGTT10350.029.3396931
GGCCCAT93350.029.3239781
GCGACCT10100.029.1944481
CGGTATG10400.028.9870551
Produced by FastQC (version 0.11.5)