FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10131267
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA697440.68840353333892No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA205790.20312365669565316No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA185220.1828201744164871No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150080.1481354701243191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTC15650.016.44700216
CTACTCG22400.013.06227637
TAGCCGG29150.013.0565732
TTAGCCG27200.012.7052721
TACTCGG22700.012.40507438
TCCTAGT23050.012.2167115
GGCGCCT17550.011.78327420
CTAGTCT22950.011.59892917
ACTCGGG23500.011.51469639
CGACTAG1550.001021812311.36086751
CTAGCCG3909.094947E-1211.2880421
GTAGTCC62300.011.26487727
CTCGGGA23050.011.16683740
GGGTTGC24000.011.0914766
GCTACTC66200.010.90011536
GCGTGGT46750.010.8233449
TGTAGTC69900.010.51218826
TAGTCCC66850.010.498157528
CGGGCGT14050.010.3343426
AGCCGGG39650.010.3201263
Produced by FastQC (version 0.11.5)