FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11076531
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA877490.792206513031923No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA385600.3481234332301332No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA352630.31835779631727656No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA283140.2556215479377072No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG32600.016.412671
TAGCCGG36550.016.2516042
GGCGCCT18700.016.23496820
CCTAGTC21600.015.17562216
CTACTCG26850.014.25669837
TACTCGG25500.014.23500938
CGGGCGT17550.014.164966
AGCCGGG47000.013.1061213
GCGCCTG27200.013.1026821
TGGCGGG27200.012.77915715
GCGCCTA2951.4188117E-1012.67771221
GCTACTC75850.012.64575936
GGGCGCC17950.012.62370319
GTGGCGG28150.012.5041914
GTAGTCC74300.012.25816627
CTAGTCT26250.012.23594517
GGTGGCG41300.012.14503513
ACTCGGG29100.012.09597839
TCCTAGT27650.012.01422615
GGGTTGC27650.012.0142266
Produced by FastQC (version 0.11.5)