FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10855977
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA833170.767475833819471No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA369430.3403010157445986No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA336790.31023462927380924No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA273930.2523310430742438No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGC500.002573632622.011941
GGCGCCT21550.016.94638420
GCGCCTG25800.016.45711921
TAGCCGG37550.015.701572
TTAGCCG35100.015.6153091
CCTAGTC20650.014.70196116
CGGGCGT18000.014.5442286
CTACTCG26300.014.30396937
GGGCGCC17600.014.24978719
TACTCGG26650.013.86846238
AGCCGGG46900.013.5563023
ACTCGGG27850.012.95492439
GCGCGCC2401.664921E-812.83314219
CTCGGGA27100.012.66401940
GCGTGGT53750.012.6472549
GGGTTGC25900.012.48636
GGTGGCG40550.012.47823713
GGCGTGG59550.012.4498578
GTAGTCC76050.012.38125927
CTAGTCT23900.012.24249617
Produced by FastQC (version 0.11.5)