FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11789182
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA1700.033.6518141
GCGTACT7600.033.5836941
CGGTATA15050.033.0412141
GCGCCTT25300.032.6133041
GCGACCA11500.032.3350071
GCGCCCT22900.031.8997061
GCGCCTA12900.031.895981
CGGTACA12900.031.7254141
GCGCATT12650.031.6566471
CGGCGTA2300.031.569681
CGGTATT19400.031.1899761
CGGTACG1000.030.8043541
GGGCGTT10450.030.5306321
CGGTCTT24100.030.4939371
CGGCGTT3900.030.4658431
CGGCGAT2750.030.4042971
CGGTAAT11000.030.4042971
CGGTATC10750.030.2926541
CGGACCA12100.030.1860851
GCGACTA6450.030.0197471
Produced by FastQC (version 0.11.5)