FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11598492
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA1200.034.8430251
GCGCCTT24750.034.5874021
CGGTCCT19650.032.5892221
CGGACCA11650.032.3008351
CGGTACT9600.031.8630311
CGGTATA15850.031.7943341
CGGCGAT3400.031.7147061
CGGTGTA11100.031.5222841
GCGTACT6500.031.1471271
GCGCGTT2900.031.1121081
GCGACCA11050.031.067471
CGGTACG1500.030.8085731
CGTACGG502.3599878E-630.8063142
GCGCATT12600.030.738711
GCGACTA7050.030.5900731
CGGTCTG15100.030.4588071
GGCCCAT106250.030.425411
CGGTCAT11650.030.4118961
CGGTATT19400.030.2867771
CGGTCTT24150.030.0704781
Produced by FastQC (version 0.11.5)