FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11598492
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1101800.9499510798472767No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470130.40533717659157764No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA434610.37471250572919307No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA341990.29485729696584695No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG37950.017.5164321
TAGCCGG40500.017.328222
CCTAGTC25050.016.7742116
GGCGCCT23100.016.5711920
CTACTCG31650.015.36161437
GCGCCTG31750.015.24387421
TACTCGG31400.014.71322638
CGGGCGT18850.014.70535856
ACTCGGG31150.014.47818539
GGGCGCC20600.014.41726819
CTAGTCT29900.014.3476217
AGCCGGG51950.014.2288623
GGTGGCG45450.014.18241513
TCCTAGT30750.013.87947515
GGGTTGC30300.013.7225756
GCTACTC85750.013.13567236
CTCGGGA31850.013.12388740
TGGCGGG33650.013.07559115
GTAGTCC82250.012.97251327
GTGGCGG34900.012.9224514
Produced by FastQC (version 0.11.5)