FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9567695
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA10100.032.2421461
GCGCCTT23450.031.714471
CGGTATT13550.031.3401891
CGGTCTT17800.031.0268251
GCGCATT9750.030.9171561
CGGCGTT4150.030.7513031
GGGCGTT10050.030.6510661
CGGTACT7400.030.625921
CGGTACA8850.030.331941
CGGTATC7350.030.2355351
GCGCCTA11500.030.0389961
GCGTCTT15800.029.9409081
GCGCCCT22550.029.7602731
CGGTCCT17500.029.6727351
CGGTAAT9450.029.5702841
GGCCCTT106800.029.2550411
GCGTACC4550.029.0150571
CGGACCA9750.028.8861031
GCGTCCT17150.028.6104281
GCGACTT8700.028.5787221
Produced by FastQC (version 0.11.5)