FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9404445
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTT21450.033.037141
GCGCCTA10150.032.957281
CGGTATA10600.031.9733921
GCGACTT7800.031.6007421
CGGTCTT20250.031.299781
CGGTACA9450.031.2066291
GCGCGTA1700.031.0696371
GCGACTA5350.030.44051
CGGTACT6800.030.0987111
CGGTCCT19350.030.0259531
GCGTCTT15100.029.8779471
GCGACCA8650.029.513161
GCGCCCT19900.029.4172741
CGGTCCA11150.029.4093381
CGGTCTA7500.029.3435441
CGGTATC7150.029.2409441
GCGCATT11450.029.2154081
CGGTAAT8900.029.1786961
GCGCGTT2950.029.0948721
GCGTACT5600.029.0815491
Produced by FastQC (version 0.11.5)