FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9129760
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA694970.7612138763779114No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA207520.2273006081211335No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA188180.20611713779989832No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA143950.15767117646027937No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTC17700.019.01661316
GGCGCCT18350.018.10322220
TTAGCCG32100.017.9698471
TAGCCGG35650.017.1555652
CTACTCG25350.015.9681637
CTAGTCT21800.015.74284217
GCGCCTG26100.015.67788421
TACTCGG25150.015.65777438
GGGCGCC18850.015.40556419
TCCTAGT22650.015.34630815
GGGTTGC24650.014.7259066
ACTCGGG26750.014.14554539
GGTGGCG39650.013.81564413
AGCCGGG47350.013.70623
GTAGTCC72450.013.63398927
CTCGGGA26150.013.54469540
CGGGCGT17050.013.4191186
TAGTCCC76000.013.17081928
GCTACTC73050.013.13050136
CAGCTAC119350.012.73710234
Produced by FastQC (version 0.11.5)