FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8930167
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCG1750.033.9512181
GCGCCTT22750.033.7577631
CGGTACG1400.033.008131
GCGCGTA950.032.429041
GCGCCCT19300.031.5829031
CGGCGAT2250.031.2965951
GCGCGTT2250.031.2965951
CGGTATT14150.031.1030651
CGGTACT6600.031.0076351
CGGTACA8950.030.9796941
GCGTCCT16300.030.510581
GCGCATT8950.030.4879531
CGGTATG8700.030.35231
CGGTCTT19400.030.2858091
CGGTATC6850.030.1972181
CGGCGTT3800.030.1126791
CGGTCCT17200.030.0655421
GCGTCTT16850.030.037071
CGGTATA10500.029.7597081
GGCCCAT79450.029.4975091
Produced by FastQC (version 0.11.5)