FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7691120
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA403780.5249950592371463No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA105340.13696314710991375No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98030.12745867962013335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG25500.016.305812
TTAGCCG23350.015.8403641
TACTCGG17950.015.07494638
CGGGCGT13600.015.0438566
CTACTCG18150.014.78762137
GGCGCCT15000.014.51974820
CCTAGTC15500.013.34170216
GCGCCTG20850.013.29473221
AGCCGGG34200.013.2512313
GTAGTCC52150.012.86650727
ACTCGGG21000.012.5712139
GGGCGCC14400.012.52755919
GGTGGCG30750.012.44856313
CTAGTCT16350.012.37898917
GCTACTC53150.012.1691236
TGTAGTC60650.012.11520526
GCGTGGT36950.012.0863929
TAGTCCC55950.012.07128128
GGGTTGC17750.012.0223266
TGGCGGG24000.011.82479415
Produced by FastQC (version 0.11.5)