FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10085101
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT2050.034.347641
CGGTATA13250.033.8777921
GCGCCTT20500.033.703621
GCGCCCT14950.032.2332231
GCGCCTA11550.032.0057561
GCGACCA9700.031.758291
CGGTGTA11950.031.6709671
CGGTATG11200.031.630691
CGGTACA12700.031.5332281
GCGTACT7000.031.119881
CCGTACG857.2759576E-1231.0644131
CGGACCA9650.030.7827381
CGGTCTG12900.030.7031941
GCGTCCT13650.030.6282221
GCGACTT9350.030.5937391
GCGTACC4400.030.5054861
GCGTCTT16600.030.4874111
GCGCATT10150.030.3502851
CGGTGTC11850.030.2670461
GCGCGTC1900.030.1106781
Produced by FastQC (version 0.11.5)