FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10085101
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1527711.514818741031944No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA374860.3716968228677135No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA325850.32310038342699793No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA245730.24365645916684425No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG34600.018.31178538
CTACTCG36300.018.18146737
ACTCGGG33600.017.28538139
GGCGCCT28000.017.28538120
CTCGGGA31550.017.01393540
CCTAGTC21800.016.95380216
TTAGCCG42300.016.864871
GCGCCTG36000.016.80523121
TAGCCGG45900.016.3920042
GGGCGCC24650.015.97535119
TGGCGGG35550.015.96593915
TCGGGAG34250.015.80115541
GGTGGCG51750.015.26154213
GTGGCGG38800.014.57188414
GCTACTC102800.014.48804836
GGAGGCT139500.014.4613744
GGCTCGA3700.014.26999631
GGGTTGC25550.014.2071636
AGCCGGG53550.014.0912313
TCCTAGT28700.013.56768315
Produced by FastQC (version 0.11.5)