FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9893286
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA1250.035.2096061
CGGTATA13300.033.4226571
CGGTACA10800.033.4165271
GCGTACT6400.032.6651651
GCGCCTT19350.032.4119451
CGGTCCT13250.032.3861961
GCGCCTA10250.031.7745271
CGGTATC9200.031.0954421
CGGTATG10350.031.0422861
CGGTCTT20800.030.8930451
CGGTCGA1000.030.8084091
CGGACCA9900.030.675041
GCGTCCA11100.030.5308551
GCGTACA6450.030.3648761
GCGACCA8650.030.2741581
GCGCATA6950.030.080151
CGGTCTG13550.030.04511
GCGGGTA7400.030.0352251
GGCCCTT88300.029.9560811
GCGCGTA1250.029.9281651
Produced by FastQC (version 0.11.5)