FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10011422
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1341401.3398696009418043No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA331320.3309419980498275No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA285920.2855937947676164No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA217700.21745162675192395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG46650.019.963751
TAGCCGG52950.018.9875742
GGCGCCT32950.018.89495720
GGGCGCC27100.017.85945719
TACTCGG38700.017.5087338
TGGCGGG39500.017.37690515
CTCGGGA35700.017.19296840
ACTCGGG38800.017.18010539
CTACTCG39750.017.1015837
GCGCCTG42250.017.07898321
ACCGATC2750.016.79969813
GCGGGCG19750.016.59717217
CCTAGTC25200.016.58700416
AGCCGGG61750.016.2814883
ACGCGCC2353.6379788E-1215.91460919
GTGGCGG43800.015.871860514
GGTGGCG59850.015.84265713
TCGGGAG39200.015.82624641
CGGGCGT25250.015.7700146
GGGTTGC28850.015.6323026
Produced by FastQC (version 0.11.5)