FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12677083
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2352181.8554583889685032No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA666660.5258780746327841No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570910.4503480808637129No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA459180.3622126635914587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG40500.017.7626938
CTACTCG42250.017.18316737
TAGCCGG55000.017.0799392
TTAGCCG51250.016.5788521
ACTCGGG41950.016.41452639
GGCGCCT32550.016.35605820
CTCGGGA38400.015.92683840
GCGCCTG41050.015.75608921
GGTGGCG59750.015.05918213
CGGGCGT27300.014.9081376
TGGCGGG39300.014.83437615
CTAGCCG5050.014.820011
AGCCGGG65600.014.5881323
TCGGGAG41950.014.52659441
GCGCCTA5050.014.37595821
GCTACTC121300.014.01951536
CCTAGTC25400.013.77144116
GTGGCGG42500.013.76919914
GGGCGCC28800.013.597012519
GTAGTCC131250.013.158127
Produced by FastQC (version 0.11.5)