FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11107165
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT3200.035.7592771
CGGACCG1950.033.854941
CGGTATA13500.033.4160771
GCGCCTT22450.033.229111
CGGACCA9450.032.8339161
CGGTCTT25350.032.5528261
CGGTATG11150.032.367141
CGGCGTA2050.032.2034761
CGGTCCT16800.032.0916631
GCGTACT6750.031.623021
CGGTACT10450.031.376561
GCGTCCT16000.031.3581351
CGGTCTA9300.031.2339121
GCGCCCT18850.031.169891
GCGTCTT19500.030.8079971
CGGTCTG14600.030.4462571
GGCCCAT104950.030.2565421
GGCCCTT115400.030.186341
GCGCGAA1100.030.0077861
GCGGGTA7800.029.9051951
Produced by FastQC (version 0.11.5)