FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8956220
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1434041.6011665635725785No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA384380.42917659459012836No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330930.36949739957258754No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260460.29081465171690735No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG134220.14986233031345814No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGT952.690831E-823.1573686
GGCGCCT22250.020.17032820
GCGCCTG29250.018.50214421
TTAGCCG35600.018.3715671
TACTCGG30500.018.17663638
ACTCGGG30950.017.98343739
TAGCCGG38950.017.90492
CTACTCG30850.017.75648337
CCGGTCG1254.862213E-717.5996025
GGGCGCC19200.017.30169119
CCTAGTC24350.017.16593616
CTCGGGA29350.017.08990140
TGGCGGG29050.016.88774115
GGTGGCG41350.016.38656813
CGGGCGT19300.016.3001486
GCGGGCG15150.015.82802417
TCGGGAG31700.015.75358641
GGCGGGC25350.015.70773116
GTGGCGG30900.015.663074514
GGGTTGC26700.015.6550766
Produced by FastQC (version 0.11.5)