FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17823480
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA3300.036.0095831
CGGCGTA3050.034.6321681
CGGTGTA22950.033.2724721
CGGTATA23850.033.2163851
GCGCGAA3100.032.653851
CGGTACG2650.032.385981
CGGTACA23850.032.385981
GCGCGTT5250.032.2752571
CGGTATT35300.031.7308251
CGGTTAG16800.031.305951
CGGTATG17600.031.258321
GCGCATT21000.031.2273581
CGGTATC13500.030.8081971
CGGTAAT24800.030.7904511
GCGTCCA21200.030.6213591
GCGTACT11800.030.397921
CGGTCGA2250.030.319181
GCGTGTA20350.030.1701931
GCGTATC12550.030.1594221
GCGCAAC9200.030.1384541
Produced by FastQC (version 0.11.5)