FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8635610
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTC1650.036.020531
CGTACGA553.4124241E-936.019072
GCGCGTA1300.035.5587271
CGGCGTA1600.034.39461
CGGTATA15500.034.225961
CGGTATG9600.034.1653061
CGGTACA12350.033.8654561
GCGTGTA9850.033.745121
CCGCGTA850.033.6662451
GCGCCTA8250.033.6191641
CGGTGGA10300.033.3393861
CGGTAAT12500.033.282971
GCGCGTT2250.033.2634051
CGGTATT17750.033.111831
CGGTGAG13900.033.0981
CGGTCCG1400.033.0188181
CGGTGTA12150.032.9735261
CGGTAAA15150.032.6919021
CGGTCTT15650.032.632021
CGGTTCG1150.032.5402871
Produced by FastQC (version 0.11.5)