FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12423714
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACC8500.035.7289621
CGGTCGT1500.035.211151
CGGTACA17950.034.6962241
GCGTACT12900.034.6311231
GCGCGTA2000.034.11081
CGGTGTA18200.033.6150361
GCGTACA14900.033.2321
CGGCGAT4250.033.1399081
CGGTATT30050.033.028761
CGGTAAA22500.032.9615481
CGGTATA19200.032.8958321
GCGCGTT4500.032.276891
GCGTACG1500.032.276891
GCGTGTT23700.032.128321
GCGCATT15550.031.9844041
GCGTATT24050.031.8437651
CGGTATG14600.031.653861
CGGTAAT21000.031.6481171
CGGTGTG22850.031.1083181
CGGTAAG11550.031.0574531
Produced by FastQC (version 0.11.5)