FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12328407
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG1600.037.14631
GCGCGTA1900.035.9153331
GCGTACT10550.035.887881
CGGTATA18150.035.0504041
CGGTGTA17500.033.9623341
CGGTACA17650.033.9231381
GCGTACC7700.033.447751
GCGTATT22800.033.405121
GCGCGTT4450.033.14261
CGGTATT27900.033.137281
CGGTACT13200.032.685411
CGGTAAT21850.032.1374241
CGGTCCA16900.031.911791
GCGTACA13800.031.9023511
GCGCATA10000.031.6981751
CGGTATG13550.031.6786821
GCGTATA14800.031.3828621
CCGTACA9750.031.3820971
CGGTGCT21050.031.3719081
GCGTGTC15250.031.3228781
Produced by FastQC (version 0.11.5)