FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8747238
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT102280.11692833783646907Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG15000.039.012446
ACGGCGA15450.037.8761567
CGGTATA16600.036.8612861
ATACGGC16300.036.575855
CGGCGAC16100.036.0737088
CGGTCTA7950.035.4386021
CGGCGTA1750.035.2171131
GATACGG16900.034.7574
GCGTACA11100.034.503251
CGGTGTA12700.034.3158871
CGGTACA12450.033.9442021
GCGTATA12300.033.821311
GCGTATT16250.033.5917051
CGGTCCA9700.033.583331
CGGTAGT6350.033.2760121
GCGTACT7950.033.223691
CGGTATT23150.033.182431
ACCGAGA17450.032.9046916
TGATACG18000.032.755183
GCGCATA7200.032.7103351
Produced by FastQC (version 0.11.5)