FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10480197
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1006610.9604876702222296No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA165260.1576878755237139No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA146410.13970157240364853No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108350.10338546117024328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCT30000.020.16632320
CCTAGTC31850.019.68569416
TTAGCCG56300.019.239661
TAGCCGG62100.018.7404212
GGGTTGC33850.018.6525616
TACTCGG42350.018.5451438
ACTCGGG40900.018.39577339
GCGCGCC4200.018.33302119
CGCGCGC2650.018.2638418
CTCGGGA37200.018.21474540
CTACTCG45100.018.09725237
CTAGTCT35750.017.599717
GCGCCTG41100.017.55687921
CGGCGCG2400.017.4163715
TCCTAGT38800.016.8399215
CGTGGCG5100.016.82324210
GGTGGCG62250.016.6808413
TGGCGGG40550.016.54719215
TCGGGAG41150.016.35938341
AGCCGGG70750.016.3559063
Produced by FastQC (version 0.11.5)